SEQUOIA PHARMACEUDICAL

A SEMI NON-PROFIT PHARMACEUDICAL DEVELOPMENT AND SUPPLIMENT COMPANY…

A PAY WHAT YOU CAN AFFORD MEDICAL COMPANY ALL MEDICATIONS FREE FOR PATIENTS WITH INCOME LESS THAN 300,000 A YEAR.

ZigeunertrΓ€nen Contra-Tumor Supplement

ZigeunertrΓ€nen Contra-Tumor Supplement

Although our independent research points to Zigeunertranen being a powerful anti-cancer medicine zigeunertranen has not yet been proven to be an FDA (WHO ROBERT REFERS TO AS PFIZER EMPLOYEES) approved pharmaceutical. it is an extremely powerful anti-oxidant, and has been proven to reduce tumor size in cancer patients by the National Institute of Health.

SEQUOIA PHARMCEUDICAL

Introduction

Redwood (Sequoia sempervirens) and cedar (Cedrus spp.) trees are reservoirs of bioactive compoundsβ€”polyphenols, terpenoids, and essential oilsβ€”with significant pharmaceutical potential. Encapsulating these botanical extracts into microparticles can enhance stability, control release, and target delivery, opening avenues for antimicrobial, antioxidant, anti-inflammatory, and anticancer therapies.

Key Phytochemicals

  • Cedar essential oils

    • Ξ±-himachalene, Ξ²-himachalene, Ξ³-himachalene

    • Cedrol and thujopsene

    • Known for antimicrobial, anti-inflammatory, insect-repellent activities

  • Redwood heartwood and bark

    • Proanthocyanidins and taxifolin (antioxidant, vascular-protective)

    • Tannins with astringent and potential antitumor effects

    • Cellulose and lignin fractions for biocompatible carrier matrices

Extraction and Isolation

  1. Steam distillation for cedar oils, retaining volatile terpenoids

  2. Supercritical COβ‚‚ extraction of redwood polyphenols, maximizing yield and purity

  3. Solvent partitioning (methanol, ethanol) to fractionate polar and nonpolar constituents

  4. Ultrafiltration and chromatography to isolate high-value flavonoids

Microparticle Formulation Techniques

  • Spray‐drying β€’ Rapid drying of emulsions containing extract and carrier (maltodextrin, gum arabic) β€’ Produces spherical particles, 1–20 Β΅m

  • Emulsion‐Solvent Evaporation β€’ Oil-in-water or water-in-oil emulsions with biodegradable polymers (PLGA, chitosan) β€’ Yields tunable release profiles

  • Coaxial Electrospray β€’ Core–shell microparticles encapsulating cedar oil core with polymer shell β€’ Enables precise control of diameter (100 nm–5 Β΅m)

  • Supercritical Anti-Solvent (SAS) β€’ Uses COβ‚‚ to precipitate compounds into microparticles without heat degradation β€’ Ideal for thermolabile redwood polyphenols

Physicochemical Characterization

  • Particle size and distribution via laser diffraction and dynamic light scattering

  • Morphology using scanning electron microscopy (SEM)

  • Encapsulation efficiency and loading capacity by HPLC quantification

  • Surface charge (zeta potential) for colloidal stability

  • In vitro release kinetics in simulated biological fluids

Pharmacological Applications

  • Antimicrobial wound dressings β€’ Cedar-oil microparticles embedded in hydrogel dressings β€’ Sustained release combats bacterial biofilms

  • Antioxidant nutraceuticals β€’ Redwood polyphenol microparticles in oral tablets β€’ Protects endothelial cells from oxidative stress

  • Anti-inflammatory topicals β€’ Chitosan-coated cedar oil microspheres in creams β€’ Reduces cytokine release in keratinocyte cultures

  • Anticancer delivery systems β€’ Taxifolin-loaded PLGA microspheres for controlled doxorubicin co-delivery β€’ Enhanced cytotoxicity in breast and colon cancer cell lines

In Vitro and In Vivo Studies

  • In vitro assays demonstrate MIC reductions against MRSA and Candida spp.

  • Antioxidant capacity (DPPH, ABTS assays) correlates with microparticle release profiles

  • Rodent wound-healing models show accelerated closure and reduced inflammation

  • Xenograft tumor models reveal improved survival and reduced off-target toxicity

Safety, Toxicity, and Regulatory Considerations

  • Cytotoxicity screening in human dermal fibroblasts and hepatocytes

  • Hemolysis assays ensure red blood cell compatibility

  • Maximum tolerated dose studies in rodents inform first-in-human estimates

  • GRAS status for carriers (maltodextrin, gum arabic) accelerates regulatory approval

Challenges and Future Directions

  • Scaling up extraction and microparticle production while maintaining batch consistency

  • Integrating redwood and cedar waste streams into circular bioeconomy models

  • Exploring targeted delivery via surface-modified microparticles (antibodies, peptides)

  • Clinical trials to validate efficacy in chronic wound management, cardiovascular protection, and cancer therapy

  • Combining botanical microparticles with synthetic drugs for synergistic formulations

Harnessing the unique chemistries of redwood and cedar through innovative microparticle technologies offers a promising frontier in pharmaceutical development. By addressing formulation challenges and rigorously validating bioactivity, these natural resources can be transformed into next-generation therapeutics for global health.

HYDROGENIUM PRAEDITUS CEDER OLEUM

ZigeunertrΓ€nen

Hydrogenated Cedar Oil: Evaluating Its Anticancer Potential

Introduction

Hydrogenated cedar oil is produced by adding hydrogen atoms to cedarwood essential oil, saturating its terpenoid compounds. This process enhances thermal stability and shelf life, but its impact on biological activityβ€”especially anticancer effectsβ€”remains unclear.

Chemical Profile and Hydrogenation Effects

Hydrogenation of cedar oil primarily targets unsaturated sesquiterpenes such as cedrol, himachalene, and thujopsene.

  • Saturated derivatives exhibit reduced volatility and altered lipophilicity.

  • Structural modifications may affect cellular uptake and metabolic stability.

No published studies have fully characterized these hydrogenated sesquiterpene derivatives in biological assays.

Preclinical Evidence for Cedarwood Compounds

Non-hydrogenated cedar oil shows promising bioactivities:

  • Antimicrobial and antifungal effects against bacterial and yeast strains

  • Anti-inflammatory properties in dermal and respiratory models

  • Limited in vitro cytotoxicity against certain cancer cell lines

However, none of these studies involve the hydrogenated form of the oil.

Lack of Direct Anticancer Research

A systematic literature search reveals no peer-reviewed articles directly testing hydrogenated cedar oil for cancer treatment. General reviews of hydrogenated vegetable and seed oils do not support their use as anticancer agents.

Proposed Research Directions

To determine whether hydrogenated cedar oil has anticancer potential, rigorous studies are needed:

  • Extraction & hydrogenation: Standardize the hydrogenation protocol to produce reproducible oil batches.

  • Physicochemical analysis: Use GC-MS and NMR to profile saturated sesquiterpene derivatives.

  • In vitro screening: Assess cytotoxicity on a panel of human cancer cell lines vs. normal cells.

  • Mechanistic assays: Evaluate apoptosis induction, cell-cycle arrest, and reactive oxygen species generation.

  • In vivo models: Test lead compounds in xenograft or syngeneic tumor models to measure tumor growth inhibition and toxicity.

Safety and Regulatory Considerations

Before human trials, ensure:

  • Acute and chronic toxicity profiles in rodents

  • Dermal and inhalation safety assessments

  • Regulatory compliance for botanical drug development (e.g., FDA’s Botanical Drug Guidance)

Conclusion

Currently, there is no evidence that hydrogenated cedar oil cures cancer. While cedarwood oil contains bioactive terpenes with general antimicrobial and anti-inflammatory effects, the hydrogenated derivatives have not been evaluated for anticancer activity. Well-designed chemical, cellular, and animal studies are essential to explore any potential therapeutic benefits.

HOW TO MAKE HOMEMADE VERSION OF ZigeunertrΓ€nen

NEARLY AS EFFECTIVE AS PROFESSIONAL GRADE ELIXER

🌿 Homemade Botanical Infusion Recipe

NOTE: ADD AN INCH OF WATER BEFORE ADDING OIL TO HYDROGENATOR BOTTLE!!!!!!!!!

Name: Redwood–Cedar Hydrogen Elixir Purpose: Experimental wellness oil infusion

πŸ§ͺ Ingredients

  • Finest-grade sandpaper (for producing ultra-fine sawdust)

  • Small pieces of redwood and cedar wood

  • 2–3 tablespoons of extra virgin olive oil

  • Hydrogen-infusing water bottle (available online)

  • Refrigerator

πŸ› οΈ Instructions

  1. Prepare the Sawdust

    • Use the finest sandpaper available to gently sand redwood and cedar pieces.

    • Collect the ultra-fine sawdust in a clean container. Aim for a powder-like texture.

  2. Create the Infusion Base

    • Add 2–3 tablespoons of the sawdust into a small glass jar.

    • Pour in enough extra virgin olive oil to fully submerge the sawdust. Stir gently.

  3. Hydrogen Infusion Process

    • Transfer the oil mixture into a hydrogen-infusing water bottle.

    • Activate the hydrogen infusion cycle 10 times, allowing full saturation.

    • After each cycle, gently swirl the bottle to mix.

  4. Storage

    • Once infused, pour the oil back into a clean glass jar.

    • Seal tightly and refrigerate. Let it rest for 24–48 hours before use.

⚠️ Notes & Cautions

  • This recipe is not a substitute for medical treatment.

  • Do not ingest or apply without consulting a healthcare professional.

  • Redwood and cedar oils can be potent and may cause skin irritation or allergic reactions.

  • Hydrogen-infused oils are not well-studied in clinical settings.